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I have some historical ONT runs that were basecalled with these models:
run | sample | base_call_model
-- | -- | --
4031P | A-1 | 2021-05-05_dna_r9.4.1_promethion_384_dd219f32
4092P | A-1 | dna_…
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Hi, remora developers,
When I attempted to use the Kmer model for the R9.4 DNA ONT sample, I had familiar trouble with the command parameter:
`remora dataset prepare ----refine-kmer-level-table`…
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Hello, Unfortunately i cant seem to make tailfindr work. Do you maybe see what the problem could be in my log?
Thank you very much in advance
Aime Picornell
── Started tailfindr (version …
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Hi,
We are interested in the function of **raw signal analysis** with Remora. Specifically the command **analyze plot ref_region**.
Becasue we are new to handling this type of data we first ran th…
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Hello developers!
we are planning a new sequencing for epigenetic modification detection on a bacterium. I have some doubts regarding the protocol because it is not clear in the pipeline provided by …
BioRB updated
9 months ago
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I am getting average Q5 reads from my 4kHz dorado-0.3.4-rc2. I had similar problem with 0.3.2
The command line
```
./dorado duplex -t 96 -v dna_r10.4.1_e8.2_400bps_sup@v4.1.0 ./pod5/split_by_ch…
ymcki updated
2 months ago
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### What happened?
Thank you for updating the workflow. I am running the workflow epi2me-labs/wf-human-variation 1.4.0 on EPI2ME Labs. I have tried to use the UCSC human reference downloaded from:
…
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I am trying to use Dorado for basecalling of a Direct RNAseq dataset prepared with the SQK-RNA002 kit. Using the only available RNA model for Dorado (rna003_120bps_sup@v3) I get the error: [error] Sam…
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Hi, @friend1ws
Thanks for developing this great tool! I've come across a base quality cut-off feature for different settings (`--qv10`, `--qv15`, and `--qv20`). If I'm understanding correctly, does…
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### What happened?
A bug happened!
I am trying to run the wf/basecalling with the new models for RBK-114 with v14 flongles
I am getting this error.
-- Check '.nextflow.log' file for details…