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I was having this issue with nanopolish and was pointed to the fact that it does not support r10 flow cells. I then tried f5c. The output here is a little more verbose, but I am still ending up with t…
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Hey,
I am trying to run guppy (v6.3.7) basecalling on our HPC on a pod5 file. The job finishes without crashing after like 5 seconds. No error message thrown. No output is created.
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Hi,
I am writing here to seek your help to solve the error that I am getting while running tgs-gapcloser. I am working on a plant genome assembly with a size of 1.75Gb, and I have used pacbio (75x …
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### What happened?
I ran this command:
```
nextflow run epi2me-labs/wf-clone-validation \
--fastq /mnt/d/nanopore_data/fastq_pass \
--basecaller_cfg dna_r9.4.1_450bps_sup \
--sample_sheet /mnt…
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Hello,
I am facing a strange issue that I am not able to solve on my own and would appreciate any insight as to what I might be missing here. I have a dataset of reads from a plasmid spiked with o…
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I started dorado (0.3.4+5f5cd02) with the following command:
`dorado duplex \`
` --recursive \`
` --min-qscore 10 \`
` --verbose \`
` --device cuda:1 \`
` dna_…
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I had read [this](https://aws.amazon.com/blogs/hpc/benchmarking-the-oxford-nanopore-technologies-basecallers-on-aws/) article, and it was very good. Thanks!
Now, I wonder about the Amazon's storage…
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I ran the RNA SUP model on an experiment, and after 531,000 reads processed, it said “zsh:segmentation fault” and ended. I compiled dorado from source a few days ago, so that was the binary I was usin…
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Instance parameters
{
fastq: /mnt/wsl/docker-desktop-bind-mounts/Ubuntu/44ea73db01058139d03114c2c973956f0331ab0d0328ce6bcc907b73c518c322/5051b9327cc3758e2e77575f824b6b65db8c48aa2b4852e7b7194f7708d…
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### Operating System
Other Linux (please specify below)
### Other Linux
ubuntu 20
### Workflow Version
v.1.8.3
### Workflow Execution
Command line
### EPI2ME Version
_No response_
### CLI co…