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We have multiple datasets that we need to prepare:
# multimodal single-cell CRISPR screens:
- [ ] https://nature.com/articles/s41588-021-00779-1
- [ ] https://www.nature.com/articles/s4…
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Hi,
I am currently conducting research on ovarian tissue using 10x single-cell RNA sequencing (scRNA-seq) datasets. However, these datasets do not include oocytes, which are crucial for my study. T…
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Dear Harmony Team,
Thanks for the tool!
I was running this tool with a plant scRNA-seq data, however this resulted an warning while combining with Seurat.
I tried to use the "new object name", …
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Hi, I am trying out the `test_data` using `stereoRun_singleLane_v4.1.0.sh`. However, I failed to find the unspliced counts among the outputs. Do I need to modify `stereoRun_singleLane_v4.1.0.sh` to g…
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I have a question regarding the first imputation step using Beagle. My experience with imputation is strictly from a GWAS perspective where imputation from a large DNA array panel will result in the …
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https://mp.weixin.qq.com/s/4JmnEOvb_d8TVdWpXDDizg
ixxmu updated
1 month ago
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Dear,
I used the ICELL8 scRNA-seq platform and sequenced ~30000 cells, with each cell having 10^6 reads.
Which strategy do you suggest to quantify the splicing junction count for my scRNAseq data?…
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Hi,
May I ask how does souporcell handle situations with extremely uneven (smth like 95% of cells from individual 1 and 5% - from individual 2, or even more skewed) ratios of cells from different i…
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Hi Tao,
We have a submission for Will Greenleaf’s lab that I worked on for a while now. It’s a bit more complicated and I was wondering if we could have a call this week and we go through the sprea…
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Hi @lingfeiwang. I'm running into an issue with the dynamic inference on my own dataset that I believe is related to the subsets being created in tmp_dynamic. I have created the prerequisite files as …