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Hi,
I am trying to install a newer version of dada2 for my PacBio SMRT link sequences,
I realised that I do have dada2 version 1.2.0 and it is not doing well with these sequences,
I read through a …
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Hello I recently used dorado with dna_r9.4.1_e8_sup@v3.6 model; however, when I go to specify the model in my wf_amplicon pipeline I get an error in medaka below:
```
ERROR ~ * --basecaller_cfg: dna…
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I have a set of ~400bp amplicon ONT reads that were sequenced from a mixture of 700 closely related strains of one bacterial species. I'd like to run some error correction to reduce the errors in the …
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Nick suggests a tiered approach. Ben will do a survey and see how many classifiers there are and whether it could be done.
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Hello. I work at North Carolina State University in the Biotechnology program. We use Qiime in our metagenomics class. The students requested that I record a tutorial on installing Qiime using Virtual…
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Hello,
I have four fastq files named 18S191096-CZ_R1.fastq, 18S191096-CZ_R2.fastq, 18S191096-NEW_R1.fastq and 18S191096-NEW_R2.fastq. Also, the size of the 18S191096-CZ_R1.fastq, 18S191096-CZ_R2.fast…
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I am trying to get Greengenes2 and redbiom working in Qiime2. I am using this tutorial:
https://forum.qiime2.org/t/querying-for-public-microbiome-data-in-qiita-using-redbiom/4653
but when I try…
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Hi, @etal !
When I run CNVkit pipeline (v0.9.7) in wgs mode to make tumor analysis,
command: `$CNVKIT batch /data/D2288/D2288_t.aligned.bam --normal /data/D2288/D2288_n.aligned.bam --seq-method wg…
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Hi,
I previously posted about high amounts of reads being lost in certain samples during the chimera removal step of Dada2. It was suggested to try primer removal and this worked to a degree but no…
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On Mac, seems to have compiled correctly. But get error on the test data, I don't think it is permissions issue.
```
src/deML -i testData/index.txt -f testData/todemultiplex.fq1.gz -r testData/tod…