-
Dear experts,
I am newbie in learning canu software,
Recently I run canu with the command line: 'canu -p BC08 -d /home/rosh2/minionphage/BC08out genomeSize=80k -nanopore-raw /home/rosh2/minionphag…
-
Hello,
I am trying to assemble a lot of pacbio reads (100x) with a few (5x) minion reads. This is the command I have used. I am running on a cluster via slurm.
#SBATCH --cpus-per-task=8
#SBATCH…
-
** canu -trim **
Hello, I need your help.
```
nohup canu -trim -p dl \
-d /public/home/zjwang/de_novo_seq/3_parent/canu/trim \
maxThreads=40 genomeSize=400m \
minReadLength=20000 minOverlapLe…
-
Hello,
I am trying to assemble a fungal genome using ABruijn and I am running into issues. The genome (~130Mb) has been sequenced on a PacBio Sequel machine with 4 SMRTcells. I ran abruijn but I ra…
-
Hi there,
I'm trying to use canu to assemble a funny marine worm, which I know is a.) pretty heterozygous and b.) contains within the library some interesting bacterial reads (possibly its microbio…
-
Hello,
I'd like to start out by saying I really like Flye. It's great, and the latest improvements have been great.
I am however having some trouble with some of my trickier genome assembies.
`…
-
My first time trying out Neptune. I have two fasta files in the inclusion directory and two fasta files in the exclusion directory. For some reason, it says `No such file or directory` at the end bu…
-
Hello,
I find arriba very attractive for rna fusion.
https://github.com/suhrig/arriba
Should I try to add this myself or it's better to request to bcbio?
Many thanks!
-
Hi,
I am assembling from ~70X coverage nanopore data with canu 1.9. I used the canu command
```#!/bin/bash
~/canu-1.9/Linux-amd64/bin/canu \
-p a_colubris_canu -d /shared/hummingbird \
java…
-
[spades.log](https://github.com/ablab/spades/files/3140574/spades.log)
[params.txt](https://github.com/ablab/spades/files/3140575/params.txt)