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Using `sc_exon_mapping` as an example: it would be convenient to be able to supply a `GRanges` object directly as `annofn=`, especially in situations where some editing of the annotation files are nec…
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https://mp.weixin.qq.com/s/sGJg54nOlEphZy1fug0ECA
ixxmu updated
2 weeks ago
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Hi Danny,
I have recently tried to install RNAFramework on a CentOS, however, I can't seem to get it to run, as every time I try to run it, it gives me the following error.
-bash: rf-map: comma…
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Hi, I want to use your isoseq toolkit to detect novel isoforms from pacbio long read RNA-seq data. But I download the data from SRA dataset. And they only offer the raw reads in fastq format. There ar…
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Hello,
I wanted to reproduce the vignette "Introduction to scRNA-seq integration" (https://satijalab.org/seurat/articles/integration_introduction)
and I ran into an issue when trying to load the…
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https://mp.weixin.qq.com/s/mBVfFlruNMLY1ZrpxQ4jQQ
ixxmu updated
3 weeks ago
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`Aligned.out.bam `and `Aligned.toTranscriptome.out.bam` are intermediate files for each sample that are generated during rna-seq's nextflow pipeline but are not used in later processes of the service.…
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Initial group info you provided:
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| Name | Department/Program | Expertise/Interests |GitHub ID |
| ------------- | ------------- | ------------- | ------------- |
| Samantha Sch…
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`samtools mpileup -r chrA:nnn-mmm` includes region `chrA:nnn-mmm` correctly, but for some combinations of BAM files it also returns pileups from the same coordinates on other contigs, for example `chr…