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Hi,
I'd be interested into getting the full length sequence of the alternate assembly (i.e. including shared sequence with the primary contig) for variant calling (e.g. with [MUMandCo](https://gith…
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This project is quite exciting, but like you mentioned in your pre-print, there is very little public training data to help optimize for this use-case.
I'd like to point the authors to a substanti…
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Minimap2 has an option "-e" to sample high-frequency minimizers, this allows to map reads that correspond to highly repetitive and nearly identical regions. Does Hifiasm use a similar technique when s…
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I extracted the local hifi and ont reads from a length of ~12Mb region， then run the verkko with default parameters. However, I obtain more than 100Mb assembled sequence, and it far exceed the length …
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Hi, @c-zhou
I run the latest `yahs` from the github issue to scaffolding a 4.5G genome, but it end with the segementation fault. Here is the command I use
```bash
# convert the merge_nodup of…
baozg updated
2 years ago
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Hi,
I have used Hapdup to make a haplotype-resolved assembly from Illumina-corrected ONT reads (haploid assembly made with Flye 2.9) and I am particularly interested in a large 32kb deletion. Here …
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Hi,
So I am trying to assemble targeted regions using Illumina-corrected ONT reads and the latest `flye-devel` version. I usually have the following command:
```
flye --nano-cor $READS -o $OUT -t…
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Hello,everyone
I am running the `run-asm-pipeline.sh.` `My command is : /data/01/user157/software/3d-dna/run-asm-pipeline.sh -r 1 M.aspalax.6.bp.p_ctg.fasta merged_nodups.txt. `The `M.aspalax.6.bp.p…
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I have HiFi + HiC, Nanopore + HiC data
The draft assembly of HiFi was finished by Hifiasm, draft assembly of Nanopore was finished by Nextdenova
The first is that the two sequencing methods, the re…
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Hi I have a quick question,
I run **hicstuff** on a _A. thaliana_ genome using both HiFi long reads and HiC long rage data which I assembled with **HiFiasm**. I got both haplotypes and for now I'm …