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I'd like to be able to design CRISPR guide RNAs with Poly. Below is a draft spec for a new CRISPR package:
## Spec
Our guide RNA design package should be lightweight and easily extendable and at…
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This is urgent. I just tried meta-mode design guideRNA for human EMX1 and EGFR, but has error retrieving sgRNA sequence? Also there's no longer a clear / refresh button, is this removed intentionally?…
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Hello, I'm new to bioinformatics and I'm trying to run this command using your program
_design.py complete-targets fasta /nfs7/BPP/Chang_Lab/buchanri/agro_crispr_dx/data/fna_assemblies/B133-95_BV2_…
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Are you adding any features for CRISPR-Csm transcript targeting systems?
https://www.nature.com/articles/s41587-022-01649-9
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**Analysis of tested guide RNA’s used for gene editing via CRISPR to predict optimal future guide sequences**
Despite the apparent simplicity of this sequence matching strategy to target the CRISPR…
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Dear authors,
First of all, thank you very much for this great work and methodology!
I have some questions to create the contrast matrix... as I understand in your example, you have two differen…
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Hi, I run this pipeline on my own phage genomes and bacterial genomes to predict interactions between them. Based on the output, it seems that only one bacterial host is predicted for each phage genom…
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Today I focused on two different areas; using bacteria and diagnostic tools and different components of genetic circuits. I already knew about the paper based circuit design but figured id include it …
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I am very interested in the development of human genetics. Genome editing, stopping aging at the genetic level and genetic modification.
**USSR Genetic**
I was surprised when I learned that in the U…
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I tryed to test CHERRY using my phage genomes, but encountered some issues.
Firstly, in the paper of CHERRY, it is mentioned that the top [n] results with a score>0.9 can be used as predictions for…