-
Hi. This is a very useful tool. I'm wondering if this tool can be applied to Parse Biosciences scRNAseq technology. Are you thinking about doing a tutorial about this? Thank you very much.
-
Hi all,
I'm using the barcoding kit SQK-NBD114.24 for sequencing on MinIon Flow cell. Before the library preparation and sequencing, I'm doing on each samples a pcr with barcoded primers:
![image]…
-
Hi there,
I have two questions:
1. You have mentioned that "ONTbarcoder has been optimized for protein coding gene like COI. While **there are ways** to obtain consensus for length variable non …
-
### Description of feature
I am currently looking into using scrnaseq for a clinical trial. In our setup we have GEX, FB, and VDJ data for several patients which are hashed using Totalseq-C. Curren…
-
Hi @huangyh09,
First of all, huge thanks for developing Vireo!
I've been testing it using a synthetic pool (3 donors), and I've noticed a high number of unassigned cells, particularly from one don…
-
Hi,
A very helpful feature to add would be the demultiplexing of the reads as an optional step. This [function](https://docs.qiime2.org/2018.11/plugins/available/demux/) has already been developed on…
-
Hi,
I am wondering if there is currenly a way to do barcode demultiplexing and duplex calling using only dorado, and if so, what would be the best way to do it?
-
Can this pipeline also demultiplex reads from cell barcodes?
-
Inquiry regarding demultiplexing of FAST5/pod5 files using Dorado
Hi there,
I recently conducted direct cDNA sequencing using a barcoded library, which resulted in the generation of FAST5 files (l…
-
Hi Luyi,
The pipeline is great! Thanks for the effort and for sharing it.
I have tried FLAMES on your published data and our own in-house data, and have two questions:
1) For match_cell_barco…