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**Submitting author:** @daylinmorgan (Daylin Morgan)
**Repository:** https://github.com/brocklab/pycashier
**Branch with paper.md** (empty if default branch): joss
**Version:** v2024.1004
**Editor:** …
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Here are details regarding barcodes into PCR products once general primer pair is determined to be viable
https://d.pr/f/K2we5f
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Hi
After an issue with our MinION Mk1C we recovered non-basecalled files into pod5 files - using the recommended `/opt/ont/minknow/bin/recover_reads` command of MinKNOW on the device.
However, …
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Currently, the guide promotes using `sampleSizeValue` and `sampleSizeUnit` to report read count information at the sample level. However, this conflicts with the recommended use of those fields to cap…
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### Ask away!
Running the below workflow options resulted in the following errror.
`Command error:
[2024-06-29 22:56:13.093] [info] Running: "duplex" "/models/dna_r10.4.1_e8.2_400bps_sup@v5.0.0" …
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# Issue Report: std::runtime_error (core dumped) with Dorado 0.7.2
## The issue:
I have basecalled my data (short read amplicon data) with several versions of Dorado and keep on having the same (…
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Hi,
We'd like to request a new term as part of the CFDE project for Kids First DCC.
new term label: linked-read sequencing assay
parent: DNA sequencing assay, OBI:0000626
definition: A DNA seq…
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Hi, Thanks for this fantastic tool!
I have been testing this tool on our pipelines and got into trouble when trying to demultiplex the 3' barcode on the second read pair.
The 5' demultiplexing w…
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Have it output common sequences people use for things like DNA barcodes. Brian's code does this already, so incorporate it into SeqScout.
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## Tissue (DNA) database of BHHU-ATRA
Dataset link: https://www.bhhuatra.com/en/tissue-dna-database
Region: Bosnia and Herzegovina
Taxon: amphibians and reptiles
Type: undefined
Why is this impor…