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When using the results of this notebook, I'll be scanning for gRNA sequences that have and **MFE** of 0, and have a relatively high **Total score**, then take those results into another program. When…
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I am wondering if it is possible to use an index built from just fasta files containing the gRNAs used in single cell pooled CRISPR screen. I can quantify gene counts using STARsolo. But if I try to u…
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Hi Aaron:
Been using FlashFry for quite a few years now. In my current project, I am designing millions of gRNAs and before we synthesize them, we filter the gRNAs based on GC% and homopolymeric se…
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[config_mApple2_gRNA_100919.txt](https://github.com/zhangchonglab/CRISPRi-functional-genomics-in-prokaryotes/files/3714579/config_mApple2_gRNA_100919.txt)
first of all, nice tool!. I have been tryi…
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python /seqprg/scripts/gene_editing/primedesign.py -f design.txt -pbs 10 11 12 13 14 15 -rtt 10 11 12 -nick_dist_max 10
2022-07-28 21:00:47,188 - INFO - Searching for pegRNAs and nicking gRNAs for …
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Hi,
I am trying to design sgRNA for campylobacter, but realised the campylobacter genome sequence is not available in the shiny app. Is there a way to upload my own genome sequence into the app? …
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Hello! I've been able to get Cast-seq to run on the G3_TOY data, but I was curious what the other files are that are called on from the G3_TOY directory. I can't find a description in the paper or in …
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Should be possible to add several guide RNAs and a single repair DNA (if any)
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dear sir,
Can SPROUT output the insertion/deletion position on the target sequence?
Just like FORECasT and inDelphi.
best
likuan
PEHGP updated
3 years ago
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Dear brocklab research group:
You did an excellent job in the field of lineage-tracing. Our research group is using the technology you developed to construct the ClonMapper library.In or…