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Hi,
I have a problem with minimap2 settings. When I try to use rb to obtain saffire file on the basis on existing paf I receive the error:
thread 'main' panicked at 'called `Result::unwrap()` on a…
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Hi Mathias,
I started to use the pipeline and analyzed successfully one nanopore dataset. In the second dataset I face quite high
CPU time consumption at minimap step. Most of the barcodes need arou…
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Hi Andrey,
I might have missed it, but is there a way to tweak the minimap2 parameters in IsoQuant? and also adding support for other aligners like GMAP and uLTRA?
Thanks
Abhijit
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### Operating System
Ubuntu 22.04
### Other Linux
_No response_
### Workflow Version
v2.10.1-g4c2c583
### Workflow Execution
EPI2ME Desktop (Local)
### Other workflow execution…
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Hello! Nice crate,
It would be nice to have the ability to build the minimap2 alignment options (i.e `--score-N`) in the same way the MapOpts or IdxOpts are currently implemented. I can see some of…
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Hi i am running mity via docker but encountered with some bam error, could not able to rectify it, actually i was trying to find out INDELS for long read ONT generated data, using minimap2 aligned bam…
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Hello,
I used the GapFiller moduel of this tool and my command is as follows:
python3 ~/biosoft/quarTeT/quartet.py GapFiller \
-d $draftgenome -g $hap1hap2.fa -t 32
and then,error follows:
[…
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I'm just trying to track down the very subtle differences in aligning a dataset between the minimap2-rs bindings and what is output by the reference minimap2 implementation.
Mostly everything is th…
rob-p updated
1 month ago
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Hi,
I am trying to follow the instruction to install liftoff but kept running into issues.
Specifically I can't make Conda to recognize minimap2/mappy.
When I did Conda install minimap2, it said no…
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Hello Mikhail,
We upgraded our OS from CentOS7 to a fresh install of RedHat 9.4 on a new HDD, reinstalled miniconda, then installed flye into a new environment. Our assemblies crash in the consensus …