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Hi,
I have assembled the mitogenomes of 8 Microctonus aethiopoides using Flye in meta mode on ONT data. The mitogenomes were extracted from the assembly using the GetOrganelle tool. According to the …
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Hi,
we recently sequenced a batch of 19 fungal isolates. I tried to assemble the genomes a few different ways (simplex + duplex or simplex corrected only) in order to figure out if there is still a…
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I recently used Novoplasty to assemble the mitogenome from short read data of _Microtonus aethiopoides_ ecotypes. Although the process completed successfully, it did not produce any contigs. I initial…
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Hi,
I tried assembling the mitogenome of my insect species using MitoZ. The final assembly is not circular, with a size of 15,442 kb. Additionally, the following genes are missing:
l-rRNA
tRNA-…
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Hi,
I am working on an HPC with Linux. I installed MitoHifi as stated and then I loaded MitoFinder 1.4.1.
I used the following code
`python MitoHiFi/src/mitohifi.py -o 5 -r CB_Parent.fasta -f c…
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Got this error:
```
Traceback (most recent call last):
File "/opt/MitoHiFi/src/mitohifi.py", line 566, in
main()
File "/opt/MitoHiFi/src/mitohifi.py", line 491, in main
plot_annot…
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Hi,
Thanks for a really cool pipeline. Super useful.
I have a series of unpublished, non-NCBI-stored mitogenomes that I want to analyze together with the mitogenomes in NCBI.
Is there a way …
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**Is your feature request related to a problem? Please describe.**
In my experience MitoHiFi has often not worked and I need to employ another strategy to assemble the mtdna (plus additional organell…
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Hello,
I have assembled a mitogenome with mitoHiFi using a reference genome from a closely related species, starting from Hifiasm contigs, I could get the final.mitogenome.fasta , then I remapped H…
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Hi Marcela!
I am trying to run MitHiFi with the testing data, the log file shows the pipeline is done, but no files were written to my working directory except for output files (.nhr, .nin, .nsq) fr…