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Hi, I user Shasta v0.11.1 to assemble a R10.4 dataset, the N50 of the assembly is only 3.4M, is this right? The dataset was download from s3://ont-open-data/giab_2023.05/analysis/hg002/sup/PAO83395.pa…
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The genome size of the species I'm assembling is 2.6Gb, and the ONT data comes from dorado sup v5.0.0 (R10).
When I used 167GB data (>Q7, >10Kb) with "Nanopore-UL-May2022.conf", I got an assembly o…
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I have a homozygous genome. so it is suggested to run the assembly with -l0. When i run the assembly with purging as 0 the N50 value is 15mb. but whwn i run the assembly with purging as 2 , the N50 va…
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Hi,
I hope you can help me out with the following. I am running scaff10x on a pacbio draft assembly and there is no change in metrics after running the software using default parameters. The pipeli…
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Plot is shaping up nicely :)
To me, the addition (longest) in the title has little meaning/added value.
The N50 has a specific definition and always refers to 50% of the total assembly length (of ea…
ssmit updated
11 years ago
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### Prerequisites
- [ X ] make sure you're are using the latest version by `seqkit version`
- [ X ] read the [usage](http://bioinf.shenwei.me/seqkit/usage/)
### Describe your issue
Thanks fo…
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Hello, I am trying to use dechat to correct ont R10.4.1 SUP reads (a mixture of N50=18kb of ~130Gb and N50=80kb ultra long of ~30Gb).
The program produces about 95Gb of recorrected.fa, then threw an …
melop updated
1 month ago
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Hi again,
I noticed that since upgrading to the v5 model, I have a lot more megabase-size reads in ULK114 data than with v4 models, albeit most of them seem to have low qualities of Q5-Q10. To put …
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just one question, for assembly-stats example (in your main page), in N50=1687656 and n=5, n correspond to L50 ???
Thanks
$ assembly-stats Pf3D7_v3.fasta
stats for Pf3D7_v3.fasta
sum = 2332801…
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Hi,
I am trying some presumably accurate Nanopore reads that I have error-corrected (see https://gist.github.com/jelber2/f22c24442c34f872d8ebf073ad721476?permalink_comment_id=5058115#gistcomment-505…