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In order to support the automatic upload of sequence data from multiplexed high-throughput sequencing (PacBio protocols in our lab specifically) we need to be able to add Multiplex Sequencing Run reco…
silby updated
4 years ago
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`samtools ampliconstats` verifies if the PCR products aligned to the reference originate in the primer regions. I want to get a list of readnames which failed this QC check. The list of those passing …
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Hi
It would be cool if there was a wiki section that would include the entire approach (including the wetlab part) of training new models. e.g. what would be the best experimental design to train m…
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It would be nice if vsearch could search for PCR products given primer sequences, as the usearch `-search_pcr` function does. The `search_oligodb` command probably does the heavy lifting and would nee…
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We need to do a bead size selection clean up to remove primer dimers from our pooled PCR product for ITS2. Details of the amplification and prep for these samples are in my [notebook post](https://ahu…
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suggest to index host-pathogen interactions from PREDICT PCR TestsGlobal Health Security in Development (GHSD)
[View Data](https://data.usaid.gov/Global-Health-Security-in-Development-GHSD-/PREDICT-P…
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Example:
`(1/1) Processing cutsite: Genome:ensembl_GRCh38_latest, Chr:8, cut_coordinate: 21919773
[Warning] No primers found, relaxing criteria, iteration=1
[Warning] No primers found, relaxing c…
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see https://github.com/ietf-rats-wg/draft-ietf-rats-corim/wiki/Composite-device-description-using-domain-membership-triples
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Hi,
In version 0.15.0 seqkit amplicon only keeps one amplicon (the largest) per primer pair. I don't always care about the largest amplicon.
Would it be possible to implement the feature of kee…
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### I raise this change request because...
- [ ] the ILCD+EPD format lacks compliance with regualtions.
- [X] the ILCD+EPD format lacks conformaty with standards.
- [ ] the ILCD+EPD format needs to b…