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Hi,
Has anyone tried Demuxlet on single nuclei using 10x? Does it work?
Also, what are people using for genotyping of human samples?
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Hi!
I am trying to compare mean and noise values between two datasets, one is single cell and one is single nuclei. both runs were performed on the same platform, with identical conditions, and the s…
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- I am an ELNer/ ELN admin
Please add 1H{19F} fluor decoupled 1H-NMR as Type ( Chemical Methods Ontologly) in the Analyses Tab. It's not listed yet.
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I am trying to analyse RNA-velocity for single-nuclei RNA sequencing data from 10X platform. I am not sure in detail how it calculates the unspliced/spliced rate when I run the command 'velocyto run10…
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Hi,
I have been meaning to try this tool for some time now. We do a lot of single-nuclei RNA-seq, as a result we end up capturing lot of reads from the pre-mrna . In order to deal with that , 1…
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Hi
That's a great method! I'm wondering if, without the reference data and positive/negative markers (I only have the DAPI image and transcripts.csv), this method is still viable for cell segmentati…
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I am working with a large single-nucleus ATAC dataset (150k nuclei, 360k peaks), but have gotten stuck at the "Caching data to disk" section of the "Atlas-level integration" [tutorial](https://mira-mu…
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### Please describe your feature request:
The task is about investigating parallel execution of Integration/Functional Tests that are now executed sequentially leading to long waiting time.
As most …
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**Primary Wrangler:**
Marion Shadbolt
**Secondary Wrangler:**
Enrique
**Associated files:**
Google Drive:
https://drive.google.com/open?id=1gnB0anWGADLlwhGHLBQb3AowIpB8145p&authuser=msha…
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Installed on a kali linux system and it started this and assumed it was because Kali, so i installed a fresh Ubuntu box and getting same results.
When running the first scan example, i dont really …