ChiSurf

ChiSurf is a software package for the analysis of complex fluorescence data acquired in time-resolved single-molecule and ensemble fluorescence experiments. The main feature of ChiSurf is the joint (global) analysis of multiple datasets. ChiSurf GUI

History

The original of ChiSurfs was to estimate errors of model parameters of fluorescence intensity decays in Förster-resonance energy transfer (FRET) experiments for FRET-driven structural models of protein and other biological macromolecules. It started as a collection of python scripts. With time more features were added, e.g., the analysis of correlation curves, correlation of time-tagged-time resolved (TTTR) data. A graphical user interface makes these scripts and tools available for users without programming experience. Overall, ChiSurf is highly experimental and its core is still heavily refactored. Consequently, features, which worked in old versions, may be not supported in newer versions, unless users explicitly demand these functions.

Features

General features

Scripting interface and open API
Interactive analysis of multiple datasets
Combined analysis of different experimental techniques

Global analysis

Analysis of multiple data sets by joint model function freely defined by the user
Freely definable models for FCS analysis & adaptable models for fluorescence decays analysis
Fluorescence decay analysis
Global analysis of multiple fluorescence decays
Generation of fluorescence decay histograms based on TTTR data
Analysis of time-resolved anisotropy decays
Analysis of FRET quenched fluorescence decays by physical model functions

Fluorescence correlation spectroscopy

Analysis of FCS curves
Correlation of TTTR-data by efficient correlation algorithms

Simulation of fluorescence observables

Kappa2 distributions based on residual anisotropies
Simulation of fluorescence quenching in protein by aromatic amino acids
Simulation of FRET rate constant distributions based on accessible volumes

Download

ChiSurf is available as a compiled Windows installation file. By downloading and using ChiSurf, you agree with the following terms:

ChiSurf is provided “as is” without warranty of any kind, express or implied. The authors of ChiSurf shall not be, in any event, be held liable for any claim, damages or other liability arising from the use of ChiSurf. As the user, you are not allowed to redistribute any or all of the code, in any manner to other parties. The downloaded code is for your use only. ChiSurf is provided free of charge to both academic and commercial users.

Conda

Either clone the repository and install dependencies using the provided environemnt.yml file. Or install chisurf using conda/mamba:

conda create -n chisurf
conda activate chisurf
mamba install chisurf -c tpeulen

To start using the software activate the chisurf environment and start the ChiSurf GUI:

conda activate chisurf
chisurf

Windows & macOS

All past and current release can be downloaded from https://www.peulen.xyz/downloads/

Linux

Linux users with snap scan simply install ChiSurf using snap.

Support

Please submit feature requests, questions, and bugs as GitHub issues. General questions are addressed and discussed in the Discord group

References

Peulen T, Opanasyuk O, Seidel C. Combining Graphical and Analytical Methods with Molecular Simulations To Analyze Time-Resolved FRET Measurements of Labeled Macromolecules Accurately. J Phys Chem B. 2017;121(35):8211-8241.
Wahl M, Gregor I, Patting M, Enderlein J. Fast calculation of fluorescence correlation data with asynchronous time-correlated single-photon counting. Opt Express. 2003;11(26):3583-3591.
Sindbert S, Kalinin S, Nguyen H, et al. Accurate distance determination of nucleic acids via Förster resonance energy transfer: implications of dye linker length and rigidity. J Am Chem Soc. 2011;133(8):2463-2480.
Kalinin S, Peulen T, Sindbert S, et al. A toolkit and benchmark study for FRET-restrained high-precision structural modeling. Nat Methods. 2012;9(12):1218-1225.

Fluorescence-Tools / chisurf

readme