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I have mainly used CRISPR/Cas9 to engineer point mutations in yeast. These are hard to follow after crossing, and we usually added synonimous point mutations that would add or remove a restriction sit…
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I was playing around with the `crispr` module and came across a weird error where the cut coordinates of a `cas9` object were way larger than the target sequence.
```
from pydna.dseqrecord import …
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#### Käsitteen tyyppi
CONCEPT
#### Ehdotettu termi suomeksi
CRISPR/Cas9
#### Perustelut ehdotukselle
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#### Aineisto jonka kuvailussa käsitettä tarvitaan (esim. nimeke tai URL)
G…
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Currently the functions do not have docstrings, and it's hard to understand how to use them.
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Creating folders if necessary...
Running CRISPR array search, it may take a while...
Running CRISPR cas search, it may take a while...
Working with 5 files
Working with file 1 - TARA_Cas9…
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Hello,
I am running your tool from the command line using the following command:
perl RD-build.pl -m cas9 -i /data/genome.fa -g /data/genome_annotation.gff -o /data/sgRNA/genomes -l crispr_test -U…
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Hi, I encountered such an error! Could you please provide the missing file? Thanks.
```
Load the assembly pseudochromosomes that have a CRISPR array and a Cas9[¶](http://222.200.186.124:9111/noteboo…
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I am very interested in the development of human genetics. Genome editing, stopping aging at the genetic level and genetic modification.
**USSR Genetic**
I was surprised when I learned that in the U…
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Dear Ben,
Did you try the Implement of maximum-likelihood estimation for true-indels in CRISPR in Dr. Feng Zhang's method in Nature Bio-technique paper (DNA targeting specificity of RNA-guided Cas9…
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**Uncovering mechanisms of early human lineage specification by CRISPR/Cas9-mediated genome editing**
The authors knock down the pluripotent TF OCT4 by CRISPR and collect RNAseq measurements (aka […