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I'd like to be able to design CRISPR guide RNAs with Poly. Below is a draft spec for a new CRISPR package:
## Spec
Our guide RNA design package should be lightweight and easily extendable and at…
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Hi, I encounter an error at the very last step during the design of sgRNA for CRISPR activation.
Everything is going fine until I try to add the CRISPRai scores.
After typing the command line in R…
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This is urgent. I just tried meta-mode design guideRNA for human EMX1 and EGFR, but has error retrieving sgRNA sequence? Also there's no longer a clear / refresh button, is this removed intentionally?…
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From #7 we see that ZBTB16 & SLC39A1 are strongly anti-correlated in ORF data and CRISPR data, and it's novel (not seen in Evotec KG).
Zinc finger and BTB domain-containing protein 16
SLC: Zinc t…
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Are you adding any features for CRISPR-Csm transcript targeting systems?
https://www.nature.com/articles/s41587-022-01649-9
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Hello, I'm new to bioinformatics and I'm trying to run this command using your program
_design.py complete-targets fasta /nfs7/BPP/Chang_Lab/buchanri/agro_crispr_dx/data/fna_assemblies/B133-95_BV2_…
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From: Anne Carpenter
Date: Fri, Dec 1, 2023 at 2:44 PM
Hi Ardigen/Ksilink,
Using this attached plot from Ardigen of the top 25 most anti-correlated pairs, I saw MYT1-RNF41.
So, I found someon…
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**Analysis of tested guide RNA’s used for gene editing via CRISPR to predict optimal future guide sequences**
Despite the apparent simplicity of this sequence matching strategy to target the CRISPR…
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Dear authors,
First of all, thank you very much for this great work and methodology!
I have some questions to create the contrast matrix... as I understand in your example, you have two differen…
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Hi, I run this pipeline on my own phage genomes and bacterial genomes to predict interactions between them. Based on the output, it seems that only one bacterial host is predicted for each phage genom…