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### Operating System
Other Linux (please specify below)
### Other Linux
Ubuntu 20.04.4
### Workflow Version
v1.3.0
### Workflow Execution
EPI2ME Desktop (Local)
### Other workf…
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With https://github.com/fulcrumgenomics/fqtk/pull/30 (and release `v0.3.0`) `fqtk` allows `N`s in barcodes.
I tried to run demultiplexing accepting any sequence for a sample (with a barcode contain…
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Hi. This is a very useful tool. I'm wondering if this tool can be applied to Parse Biosciences scRNAseq technology. Are you thinking about doing a tutorial about this? Thank you very much.
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need to assess and have a plan of action for data triage/archiving for this output.
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Hi,
I am wondering if there is currenly a way to do barcode demultiplexing and duplex calling using only dorado, and if so, what would be the best way to do it?
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Hi all,
I'm using the barcoding kit SQK-NBD114.24 for sequencing on MinIon Flow cell. Before the library preparation and sequencing, I'm doing on each samples a pcr with barcoded primers:
![image]…
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Hi there,
I have two questions:
1. You have mentioned that "ONTbarcoder has been optimized for protein coding gene like COI. While **there are ways** to obtain consensus for length variable non …
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### Description of feature
I am currently looking into using scrnaseq for a clinical trial. In our setup we have GEX, FB, and VDJ data for several patients which are hashed using Totalseq-C. Curren…
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Can this pipeline also demultiplex reads from cell barcodes?
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Hi @huangyh09,
First of all, huge thanks for developing Vireo!
I've been testing it using a synthetic pool (3 donors), and I've noticed a high number of unassigned cells, particularly from one don…