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Hello,
I encountered the following issue while running the run_calibration_check() function:
```
sceptre_object
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I'd like to be able to design CRISPR guide RNAs with Poly. Below is a draft spec for a new CRISPR package:
## Spec
Our guide RNA design package should be lightweight and easily extendable and at…
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[config_mApple2_gRNA_100919.txt](https://github.com/zhangchonglab/CRISPRi-functional-genomics-in-prokaryotes/files/3714579/config_mApple2_gRNA_100919.txt)
first of all, nice tool!. I have been tryi…
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Hello,
I'm trying to understand the output for gRNA counts for periscope data applied to illumina sequenced libraries. I see gRNA counts for every ORF in my results and the counts are different per…
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Hi,
When I do the gRNA design for short sequence where only 2-3 gRNA exist, it worked very well. However, when I increased the input sequence length and there were 19 gRNA, the scores and the numbe…
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As we consider what the must-haves and nice-to-haves are for a CRISPR-Cas kit: should we be providing an expression device/plasmid for gRNA paired with a particular Cas? This may all be moot if we wan…
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When using the results of this notebook, I'll be scanning for gRNA sequences that have and **MFE** of 0, and have a relatively high **Total score**, then take those results into another program. When…
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GanttStart: 2023-04-20
### Background
In https://github.com/rasilab/github_demo/issues/3 and https://github.com/rasilab/rqc_aggregation_aging/issues/101, we identified and validated FK as a stalling…
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I am wondering if it is possible to use an index built from just fasta files containing the gRNAs used in single cell pooled CRISPR screen. I can quantify gene counts using STARsolo. But if I try to u…
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Right now we are only caching the response precomputations. It would be good to cache other things as well. For example, if the user sets the sidedness of the test, then runs the gRNA assignment, QC, …