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Hello,
and thank you for this amazing tool. I am currently examining different methods of hybrid de novo transcriptome assembly.
I constructed various assemblies from mouse data and compared their…
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Given the higher accuracy of illumina short-read SNV/ indel calls compared to ONT long-reads, does longphase support phasing with short-read SNVs and long-read alignments/BAMs?
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Dear developers,
I am currently working with miRNA-seq reads from Illumina platforms, whose length ranges roughly from 16 to 30 nucleotides.
Do you think bowtie can outperform other popular mapp…
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Hi
I can see star throwing out very short reads which in my case leads to an unbalanced output with singletons (ie throwing one read of a pair out). The reads being chucked are rubbish themselves, b…
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Hey guys,
I am struggling to find some good illustrations to:
`short reads`
`long reads`
`CNV`
`STR`
`SV`
`SNPs`
`indels`
Thank you very much for your amazing work!!
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From the short reads workflow wdl shortReads_assembly.wdl we need outasmstats, this has the assembly stats, that file needs get propagated to be part of the output of jgi_metaAssembly in jgi_assembly.…
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So one common use with websockets is to exchange JSON docs back and forth. They are self delimiting, but doing byte-at-a-time parsing can be excruciatingly slow.
json.load does this:
```
return load…
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### Description of the bug
Hi all - long time listener first time caller:
I have a rather large set of Illumina data along with some nanopore reads on which I was trying to run the hybrid assembly o…
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Thank you again for developing this great tool. We have a fish ONT dataset (10.4.1) which we assembled with nextdenovo to produce a genome size of ~560Mb and an N50=~7Mb.
I thought I should also try…
melop updated
2 months ago
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Dear @lfaino
Do you think it would be difficult to implement an option to use several bam files for short reads (many libs), instead of merging in a very huge file. Or another solution (very inter…