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1. I add `--estimate-poly-a` to the basecaller command. In the `bam` resluts, I get `pt:i` tag for some reads. But I could NOT find the ployA start and end index according to the raw signal. So in the…
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Hi,
My knowledge about LRS and ONT is mostly theoretical for now. I read several papers where the config file trained with e. coli underperformed when not using the same species, which makes total …
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Hello,
I'm having issues with the split_qscore.py script. I wanted to filtered the output from basecalling done by Dorado using this:
I completed with no error message and I got a stats.txt file lik…
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# Issue Report
## Please describe the issue:
I am getting this error when running dorado 0.3.4 with this command:
./dorado basecaller -r --modified-bases-models dna_r10.4.1_e8.2_400bps_sup@v4.2.0…
ymcki updated
6 months ago
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Bam file:
```
$ cat v02.2_trimmed_aligned_moves.sam | grep c914b199-6660-4ebf-b3ef-a946446fb90e
c914b199-6660-4ebf-b3ef-a946446fb90e 0 IntendedSequenceTop_143x51 23155 0 162M1D30M98S * 00GTAATGG…
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Hello,
I have problems getting dorado basecaller to run in the modified bases mode.
System details:
- 64GB RAM
- GPU: RTX 3090 Ti
- NVIDIA driver version: 535.113.01
- CUDA version: 12.2
- Do…
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Hi, dorado v0.53 becomes slow with official m6A_DRACH model `--modified-bases-models rna004_130bps_sup@v3.0.1_m6A_DRACH@v1` and uses heavily CPU (load over 10 on 16-core CPU) instead of GPU!
```bash
…
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Dear @cjw85 ,
Since 2022, our laboratory has been utilizing Oxford Nanopore Technologies (ONT) sequencing for bacterial isolate sequencing. Last year, ONT has introduced Dorado as a new basecaller,…
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I'm encountering errors (on an M1 Mac) when working through the quickstart guide.
Here are the lines I ran to install the software within a conda environment with python 3.10:
```
python /Applica…
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Hi William,
I tested this package, and it works. Very cool. Thanks a lot!
However, I have a few questions regarding my output.
1. I noticed that barcodes are removed during the analysis. Can you p…