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Hi,
I would like to analyse multiple samples at once after demultiplexing. As input data I have one fasta file for each sample and each amplicon.
I'm not sure how to organise my data and I coul…
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Hi there,
Have been using your library to demultiplex libraries - 384 well illumina libraries per ONT run - which have a similar construction to Tru-seq paired indexes.
The first sets tested w…
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core genes: 2017
single copy core genes: 1836
Number of conserved sequences: 1812
species specific conserved sequences: 536
potential primer pair(s): 4578
primer pairs with good target binding: 4…
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Hello!
Might have an idea for your tool:
### Prerequisites
- [x] make sure you're are using the latest version by `seqkit version`
- [x] read the [usage](http://bioinf.shenwei.me/seqkit/usag…
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### Description of the bug
Hello.
I am trying to use viralrecon as to detect variants with a minimun frequency of 0.1. Nevertheless, when using viralrecon with the instructions given below, I have …
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Hi, thanks for your contribution to the variation detection. I am using **VarDictJava**, and encountering some problems, hoping to get your help. The variant we want is not in the tbl file VarDictJava…
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Hi I've noticed there're problematic sites added for V4 as well. Great work guys!
Just wondering if we can have an additional column for the particular setting (sequencing platforms, amplicon version…
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Somewhat an extension of #75, but there have been improvements to the `uchime_denovo` algorithm of vsearch. Specifically:
- uchime2_denovo
- uchime3_denovo
As outlined in the [vsearch manual](h…
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This is one component of issue #375
## MIxS
```
bacteria_archaea
eukaryote
metagenome
metatranscriptome
mimag
mimarks-specimen
mimarks-survey
misag
miuvig
organelle
plasmid
virus
``…
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[Suggestion to add amplicon resequencing data-processing functionality, brief description of new scripts to aid in this, and request for guidance on unit testing or code review]
---
## Justification …