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Hi!
At the end of Step 7, I encounter this error:
============GFA DUMP STARTING============
Graph has 23455016 edges
Dumping edges
Dumping connections
============GFA DUMP ENDED============
…
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I am using the add_hash_with_abundance method to add pre-calculated kmers with their abundances. Can this work with a scaled minHash? Will the order or kmers streaming in matter?
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I can't seem to assemble my data, 5 Mbp bacterial genome, PE reads. I've tried various `k` and `g` etc.
```
LightAssembler -k 31 -G 5000000 -t 72 /data/R1.fq.gz /data/R2.fq.gz --verbose
```
```
---…
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Dear developers:
openDBA has many applications, which are very interesting. Nanopore provides a kmer model for generating standard nanopore signals. By providing a sequence based on this kmer model, …
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**1. What were you trying to do?**
Call variants with vg. I'm trying to align of paired-end illumina reads from different bird individuals to a vg graph generated with the Cactus Pangenome Pipe…
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Hi~,
I have got a whole new set of problems now:
![image](https://github.com/zhangrengang/SubPhaser/assets/34566522/3c44b738-630b-42b4-800b-42a7e8777de1)
there is abnormally few subgenome-s…
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Dear,
I have a diverse number of reads in a RNA-seq file. I've normalized to only 10 maximum repeated reads and it still takes a lot of RAM memory during Trinity execution:
`inchworm --kmers jelly…
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Hi, Cargo is installed and added to path however, on building the `k-mer_distinguishing` tool with `cargo build --release` the package `rust-htslib v0.22.0` fails to build resulting in a error out `e…
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I've assembled a 1.3 Gb genome with hifiasm (version 0.14 e6e6dbf), merged both haplotypes and compared the kmer content to the read kmers with kat spectra-cn.
Because of a small heterozygocity of 0.…
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Add support for matching amino acid kmers. An amino acid kmer can be represented as a nucleotide kmer where each amino acid gets mapped to a canonical (e.g. lexicographically smallest) codon.
An am…