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### Description of feature
Hi there - I'm trying to use ampliseq for 12S amplicon data and running into issues adding our own custom database b/c of incompatible formatting. It would be great for amp…
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[Suggestion to add amplicon resequencing data-processing functionality, brief description of new scripts to aid in this, and request for guidance on unit testing or code review]
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## Justification …
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Make a decision on how to use the data from overlapping amplicons.
Right now only one is used, which is an issue if they have conflicting information, and also if only 1 pool is used and the marker …
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Hello- I have followed properly the instructions provided to install this pipeline-umi-amplicon. However, the installation test failed Command used:
`snakemake -j 1 -pr --configfile config.yml`
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We need to add slot to identify a data object that points to an external data source (e.g., the EMP500 amplicon data).
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We need to handle adapter sequences.
Observed behaviour:
1. amplicon N is ok, amplicon N+1 is dropped.
2. reads at the end of amplicon N have adapters.
3. a few bp of adapter are included in the…
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I am doing 16S rna amplicon based ONT sequencing.
My target region is only of 1kb to 2kb in length.
Per sample reads is around 50K.
Will HEERO give me good error filtered estimates ?
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Hi,
I'm testing the pipeline and everything goes well until :
artic_plot_amplicon_depth --primerScheme /home/linux/programmes/artic-ncov2019/primer_schemes/ZaireEbola V1/ZaireEbola.scheme.bed --samp…
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Thank you very much for the nice tool. I was wondering how suitable is starchip for amplicon based fusion detection in RNA-seq workflows (targeted amplicon-based RNA-seq using reverse-transcribed cDNA…