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# Issue Report
## Please describe the issue:
I am working on epigenetic analysis for bacterial samples using Oxford Nanopore sequencing (FLO-MIN114), and I’m transitioning from Tombo to Dorado for…
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What are empirically good values for `l` and `k` when generating a custom database which is going to be used for Oxford Nanopore data?
Anyone some hints here? Would you stick to the default? I am a…
ms-gx updated
3 months ago
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I'm running PASTA thus:
`
python pasta/run_pasta.py --num-cpus=6 --aligner=HOMOLOGS --merger=MUSCLE --tree-estimator=RAXML -i simulated_reads/x.fa --alignment-suffix=HOMOLOGS.MUSCLE.RAXML.x -j HOMOL…
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Hi,
I have Nanopore sequencing data for the 16s gene of microbioal communities. Can dada2 be used for this type of sequencing data, and if not, is it in development? Thanks!
Best wishes,
Carla …
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Is kraken suitable for taxonomic analysis of long reads (nanopore sequences) ?
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SLOW5 is specific to Nanopore data, not generalizable to other data types. If someone want to keep large data arrays, they either need to come up with a new customized format or have to use HDF5. I wo…
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Hi team,
Firstly, thanks for this tool, it looks great and I'm excited to use it.
I'm having some installation issues, I hope you can help with these.
I cloned the repo, entered it, and in…
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Hi,
I recently used Lofreq v 2.1.5 to make variants calls to the HIV WGS data. Before running lofreq I aligned reads to BWA and filtered for properly paired alignment using samtools. After running lo…
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Hi,
I'm trying to run masurca with a hybrid assembly of illumina PE and Nanopore data and I get the following error after running the config file:
Error line 23 of configuration file 'sr_config…
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Base calling, is the systematic assignment of nucleobases to chromatogram peaks in case of electrophoresis, current changes resulting from nucleotides passing through a nanopore in case of Nanopore se…