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Hi,
I have been using bambu lately for novel isoform identification from long-read RNA-seq data these days. And it is efficient and useful.
However, an issue happened when performing novel isoform…
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### What happened?
Just trying out the workflow on my local machine with the included test data. Works fine without the approx_size_sheet.txt, but when I _do_ specify it, the workflow does not perfo…
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Hi,
I'm wondering that the input file(s) are for calculating and plotting the empirical error distribution? I'm very interested in being able to access that part of the pipeline as a standalone comma…
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### Ask away!
Hi,
I am running wf-single-cell for my ONT 10x 3' single cell data which have 140million reads. I am using the following code with memory assignment of 256Gb. However, I still got " …
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Hello there,
I am trying to use your package using nanopore samples, but I am getting the following error:
`Cannot determine format of file: /home/user/my_output/distmatrix.txt`.
The `distmatri…
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Hi Artic Team,
Would it be possible to add details of the most recent SARS-CoV-2 primer versions (including V5.3.2) to this repo as our nanopore pipeline queries this for primer details prior to da…
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Hello.
I'm working on setting up Dorado in AWS.
I'm basing my set-up based on the blog post found here.
https://aws.amazon.com/blogs/hpc/benchmarking-the-oxford-nanopore-technologies-basec…
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**Submitting author:** @lskatz (Lee Katz)
**Repository:** https://github.com/lskatz/fasten
**Branch with paper.md** (empty if default branch):
**Version:** v0.7.2
**Editor:** @kellyrowland
**Reviewer…
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### What happened?
Hello,
I tried to run the pipeline using the command line multiple times on my local linux pc. But it seems like it gets stuck at the kraken_server/kraken_client step. The demo…
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### Ask away!
I hope that someone can help me with this issue, I´m trying to convert HOMD-ref-seq and HOMD-taxa (https://www.homd.org/download#refseq) to be used with wf-metagenomics on my MAC Studio…