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Add barcodes management/demultiplexing option when performing basecalling
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Hi. This is a very useful tool. I'm wondering if this tool can be applied to Parse Biosciences scRNAseq technology. Are you thinking about doing a tutorial about this? Thank you very much.
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Can this pipeline also demultiplex reads from cell barcodes?
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Hi,
I am wondering if there is currenly a way to do barcode demultiplexing and duplex calling using only dorado, and if so, what would be the best way to do it?
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Hi Luyi,
The pipeline is great! Thanks for the effort and for sharing it.
I have tried FLAMES on your published data and our own in-house data, and have two questions:
1) For match_cell_barco…
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Support the first step of demultiplexing without appealing to CASAVA
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I'm starting to regularly analyze samples multiplexed with HTOs a la [Stoeckius et al](https://www.biorxiv.org/content/early/2017/12/21/237693) and multimodal CITE-seq ADTs. I have some rough ports o…
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In the preproc_experiment there was a flag (-f) that was used to read in the target samples. Does seqyclean have such a flag?
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Hi there,
Have been using your library to demultiplex libraries - 384 well illumina libraries per ONT run - which have a similar construction to Tru-seq paired indexes.
The first sets tested w…
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Hi,
A very helpful feature to add would be the demultiplexing of the reads as an optional step. This [function](https://docs.qiime2.org/2018.11/plugins/available/demux/) has already been developed on…