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Hi, I am working on the m6a identification on my ONT data. I installed NanoSPA according to the instructions. I can run the test data successfully and get the output files in the plus_strand folder. H…
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Project: ENCODE
So - we are working with a new High Throughput Sequencing Assay: icSHAPE, that captures RNA secondary structure.
NTR: "icSHAPE"
Editor preferred term: "icSHAPE"
Alternative…
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I have RNA modbams with m6A (in DRACH motifs) and everything runs without error but wgmeth/differential methylation results are sort of confusing - are there going to be RNA-specific additions to meth…
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Would this be possible? The YC tag colors the entire alignment, whereas the MM/ML tag only typically color individual bases.
It would be great to be able to visualise both simultaneously.
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Hi, ZhenWei:
The R package exomePeak2 is nice and ideal for m6A-Seq analysis in our group.
But recently a result troubled me:
In the Differential Modification Analysis result: DiffMod.xlsx, t…
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Hi nanom6a team! I noticed that you used m6A sites identified by miCLIP from public data (Nanopore direct RNA sequencing maps the complexity of Arabidopsis mRNA processing and m6A modification) in Fig…
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I'm trying to predict the m6A site of Arabidopsis DRS data with nanom6A, but I received the following message while performing "predict_sites". ! What files should I check for? or any other informatio…
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First hank you for providing us such an amazing tool.
Today when I used RADAR to analysis m6A-seq date it said:
There are 3299 reported differential loci at FDR < 0.1 and logFoldChange > 0.5.
When …
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# Issue Report
unbelievably slow bascalling using dorado
## Please describe the issue:
I have sequenced some samples using direct RNA-seq on promethION machine. I've been running dorado for basecal…
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Hi
exomePeak2 is so nice a better package for m6a , I installed it correctly and try to use , all bam files have been indexed. I come across the error like this :
My command is :
```
libra…