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We would like to verify whether there is a significant difference in efficiency of the hm pipeline for seq GWAS.
1. Calculate the average % of dropped and unable to harmonise variants among a repres…
ljwh2 updated
7 months ago
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Hi,
Are there reference files available to perform imputation on CHM13-v1 aligned data?
Also, would QUILT work on high-cvg (~35x) whole genome sequencing, to help find the very small number of mis…
gevro updated
2 years ago
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Dear shujun，
First of all apologize for my bad English. I have sequenced dozens species, these species belong to the same genus (eg. wild, cultivars, landrances), and I will construct a pan-genome. S…
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Hello everyone,I'm trying to conduct a mapping of my 10x scRNA-seq data with a customized reference genome using STARsolo.My analysis seemed to work properly ("finished successfully" message appeared)…
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Hello,
I am very interested in utilizing your software for a project. However, we are using Ion Torrent PGM for sequencing and thus we only have a single read library, reference genome and Denovo con…
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An anonymous user added a new forum post "most transcripts and many genes
have no reads" in thread "most transcripts and many genes have no reads" at
http://fluxcapacitor.wikidot.com/forum/t-40930…
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Hello!
I have got a de novo genome assembly from Illumina PE sequencing. This assembly has got 8.7 million scaffolds in fasta format. I splitted this fasta file to multiple fasta files. One fasta f…
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Hi,
I am wondering what's the expected runtime of simreads for 30X coverage and 300Mb genome? I generated the error profile using the top 20k reads from a Nanopore sequencing run and started the si…
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ConPair software uses 7387 pre-selected informative genomic markers based on following criteria.
SNVs (easier to genotype from sequencing data)
exonic (to allow comparison of exome and WGS samples…
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After talking with Stephan today, we thought it would be nice to have the option of getting more information about ecah package out of trident list. This would streamline integration into data analysi…