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Hi Author(s),
I think it is super cool that you've implemented this dynamic strategy!
We where trying to normalize some amplicons on a given chromosome, so we made sequences that where unique to…
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When trying to figure out how to document metabarcoding data, I came across some things that were not super clear to me.
Any insight in this question is much appreciated, and perhaps the terms could …
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Hi,
I ran q2-dbbact using qiime2-2021.4 and got the following error
Plugin error from dbbact: Duplicate observation IDs
The content of the log file is following
trimming primers if needed
…
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>>
>> https://github.com/rmcolq/pandora/blob/815da22867f2bdaa3e3bf40f382be830c1506b0a/src/estimate_parameters.cpp#L217-L231
>>
>> In particular https://github.com/rmcolq/pandora/blob/815da22867f2…
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In the PBCCSWholeGenomeSingleFlowcell workflow, in CallSVs, Sniffles task I got an error where it sounds like there's too few reads in the bam to estimate some parameter it needs? Log file excerpt fol…
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Hi Katherine,
Thank you rebar, it's working very nicely with a in-silico dataset (part of a quality assurance program in Australia).
I am getting some mixed results when I come include or exclu…
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Hello,
Executed code:
shapemapper --name example --target ../FASTA/RNA.fasta --out RNA_shapemap --amplicon --modified --R1 100mM_1M7_RNA_R1.fastq --R2 100mM_1M7_RNA_R2.fastq --untreated --R1 DM…
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See a great example here: [https://github.com/isugifNF/blast/blob/master/main.nf](https://github.com/isugifNF/blast/blob/master/main.nf).
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Hello!
I am using cutadapt to demultiplex my reads (v2.10 with Python 3.7.6).
Basically, I have a pair of fastq files (e.g. input_R1_001.fastq and input_R2_001.fastq) which contain sequences fro…
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Hi @benjjneb and community,
I'm running through the [standard DADA2 pipeline](https://benjjneb.github.io/dada2/tutorial.html) but lose >50% of my reads at the merging step. The sequencing setup was…