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Hi, thank you for putting spliceai_reforged together. Have you had any experience running it with more modern GPUs? I have a GTX 3060 Ti and have been unable to get it to work despite everything being…
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Source: GEO
```yaml
label: GSE26367
title: Protein-coding and MicroRNA Biomarkers of Recurrence of Prostate Cancer Following Radical Prostatectomy
accession: GSE26367
year: 2011
pubmed: 21703…
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I have assembled for a genome a "suspected" highly hetrozygous genome using hifi.
The assembled genome size is 8.2G, which gives following BUSCO results.
Could you please help me understand how t…
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## Bug Report
### iRODS Version, OS and Version
4.2.7
Ubuntu 18.04
### What did you try to do?
`iget` a data object which has one good and one bad replica
### Expected behavior
`ige…
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With `-so` and writing to bam (file or stdout), every read gets a `QS` tag, including duplicates. But when writing to sam (file or stdout), it looks like reads marked as duplicates are missing the `Q…
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HI,
If I would create an Illumina assembly then could I combine the Illumina assembly contigs together with pacbio reads and run Racon, miniasm, and minimap as a hybrid assembly?
Thank you in adv…
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Apparently Illumina uses the Q=2 quality score as a "Read Segment Quality Control Indicator". I can't find any recent mentions of this on Illumina's website. One document from 2011 describes it as:
>…
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Vsearch supports --fastq_join in which "sequences are not merged as with the fastq_mergepairs command, but simply joined with a gap."
**Addition Description**
It would work like the existing [me…
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Hello again!
I want to process the sequencing data obtained by Croce _et al_ in **"Phage display profiling of CDR3β loops enables machine learning predictions of NY-ESO-1 specific TCRs"** with TRUS…
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I am assembling a Drosophila genome with Illumina paired end , Illumina mate pair reads along with nanopore long reads. Size of the genome is ~190 MB.
Find the necessary files here
[params.txt](http…