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Hi! I have used this software in the past to determine different haplotypes across short gene malaria markers with illumina data. However, I am currently working on this project that involves nanopore…
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Dear Michael A. Boemo,
Thank you for this tool.
Pretty new to Nanopore sequencing; I seem to have run into a few issues whilst trying to use DNAscent starting on fastq files generated during th…
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Computer: mac osx
My quest for getting things to work:
First, a non-clang compiler, couldn't figure that out:
```
make CC=/usr/bin/gcc
Cloning into '/Users/smwaters/canu/src/utility'...
War…
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Hi,
I've just installed CANU and I'm trying to assembly one MiniON run with the command:
canu -p uncorrected_reads -d ./uncorrected_reads genomeSize=2.1m maxMemory=10 maxThreads=2 -nanopore-raw /scra…
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Hi,
I am trying to assemble two short, partially overlapping PCR fragments (6.8 kb + 2.2 kb). The fragments have been mixed equimolarly and sequenced on a Nanopore MinION system (LSK109 ligation li…
scogi updated
4 years ago
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Hi, I am not sure which value I need to give here to configure pipeline..Please suggest...
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A conda environment named MetONTIIME_env is created, where seqtk, porechop, pycoQC, Nano…
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Are there plans to package argopt in Conda? And if not, do you mind if I take a stab at it?
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Hi ,
I try to have flair running on our Nanopore data.
I aligned reads using minimap, converted bam to bed 12 then ran the flair correct script with
` python flair.py correct -c /mnt/BIG/MINI…
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Hello,
I am running Canu1.6 on a remote cluster to assemble a 27 Mbp protist genome with about 100x coverage.
**My script is below:**
```
#!/bin/bash
#$ -S /bin/bash
. /etc/profile
#$ -cwd…
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Dear Micheal,
porechop step gets killed after 2 hrs start of analysis. I have tried to do Analysis for 28 Gb fastq.gz of M.Tb reads data on PC with 16 Gb RAM, 1Tb storage. I have tried to reinstall…