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This is just to document an error that @JeffXiePL came across in this notebook:
https://github.com/JeffXiePL/pydna/blob/docs_peilun/docs/notebooks/CRISPR.ipynb
You were trying to instantiate a c…
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Hi,
Does the capTEs suitable for nanopore direct rna sequencing data?
Thanks,
Bai
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Hello,
In the paper, it states: "**During pretraining, Evo uses an effective vocabulary of four tokens, one per base, from a total vocabulary of 512 characters. We use the additional characters to …
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Hi,
I have run the vignettes from github and also procedure 3 from the paper. Everything works great until I run "score_combn_batch". Then for the dataset from the vignettes (Cas9 vs. Cas12a) this …
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I am writing to you because I am collaborating in a CRISPR/Cas9 knockout screening analysis. We are using the GeCKO v2 gRNA library and we found that a huge number of gene symbols are outdated. I foun…
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Hello,
I am running your tool from the command line using the following command:
perl RD-build.pl -m cas9 -i /data/genome.fa -g /data/genome_annotation.gff -o /data/sgRNA/genomes -l crispr_test -U…
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Hello,
I'm trying to get guide RNAs out of CasOligo. In Supplemental file Figure S4 several guide RNAs are listed. Is it possible to get the sequence of these guide RNAs from CasOligo? For examp…
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This happened when @GC-repeat tried to import two Cas9 nickases in the CRISPR-Cas package.
Although everything was in order and several parts were already in the Cas variants library, the newly ad…