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One possible filter for the gRNA design tool is to avoid sequences with 4 identical nucleotides adjacent to each other ("AAAA" etc.). Possibly other similar filters are useful too, after doing some in…
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https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8262757/
I'd like to get the plannotate auto annotation suite working with poly.
Here's a link to the code: https://github.com/mmcguffi/pLannotate/t…
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Hello!
When ReCo accesses a library for paired sample type, I encounter an error where all of my samples are considered invalid. Below is the error presented:
```
2023-08-29 14:05:07 WARNING: Du…
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Hi everyone,
I have an issue with the CRISPResso analysis of some of my NGS samples (Input: paired end fastq files, amplicon sequence and gRNA added).
The goal is to see the editing efficiency…
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貼吧活動:(請查閱 [SARS-CoV-2 Timeline by 2020.02.21](https://github.com/agorahub/_meta/blob/agoran/theagora/sari/Memorandum_2020-02-21_SARS-CoV-2-Timeline_Nathan.pdf?raw=true), by Nathan :cloud: )
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Hi-
I was curious if you could help me understand what happens in the case of multi-mappers, ie a given sequence that appears multiple times in the same reference. In your example with 4,546 Salmonel…
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Hi,
I am Jiseok Lee from Dr. Hyejung Won's lab in UNC. I watched your IGVF seminar about low MOI sceptre and it was very, very helpful. Thank you!
I have CROP-seq data and I tried following your…
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Hi,
We are currently working on identifying gRNA sequences targeting sub-families of transposable elements. The purpose is to test "consensus" sequences that we identified to show that these sequen…
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Hi @LihuaJulieZhu ,
Hope you are doing good, I wants to know how to run Guideseqanalysis function for multiple gRNAs, In document it is return that the
gRNA.file file containing one or more gRNAs in…
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I'm running into an issue where my iGUIDE processing job fails at the very last step. I've run into a similar issue before and solved by removing samples with no reads assigned (see [this](https://git…