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Hi everyone,
Many thanks for developing this pipeline and the UMI strategy, this is really promising! I am trying to implement the pipeline to analyze some UMI data we have been sequencing with Na…
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Hi,
We are trying to access the ground truth but the link is not accessible. (https://www.zymoresearch.eu/media/amasty/amfile/attach/_D6300_ZymoBIOMICS_Microbial_Community_Standard_v1.1.3.pdf)
C…
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Hi Guys,
I am analysing a 16s amplicon data containing a mock sample from ZymoBIOMICS. I have the sequence of the 8 genus of bacteria contained in the mock sample from the suppliers. I was able to …
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We obtained full-length 16S rRNA sequencing data of ZymoBIOMICS Microbial Community Standards using Sequel according to the manufacturer's instructions.
https://www.pacb.com/wp-content/uploads/Proce…
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Hi,
Currently the definition for the attribute "sample material processing" is
> Any processing applied to the sample during or after retrieving the sample from environment. This field accepts O…
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Hi, I try to build my SBT database, but I get some problems.
There is my command below:
1. I use `computes` to saves signatures for NCBI sequence.
`find . -name '*' | xargs sourmash compute --outp…
J-I-P updated
3 years ago
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We have included a mock community sample in our analysis.
This mock comprises 8 species.
Dada produces 273 ASVs for this sample.
When we cluster the ASV sequences with decipher they form 8 well def…
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Hi
I have tried all possible versions of Silva 138 V3-V4 classifiers generated using three different dereplicating mode "uniq" "majority" and "super" in RESCRIPt to classify 16s sequences for mock…
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Dear,
I head a talk today, and the speaker introduced the R10, which is much more accurate than R9.4. I am interested in the R10 data and found this github site. I wonder what is the species of …
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Hi! Not sure if this is a bug or an artefact of the method:
I have 16S data and also mock community samples. I have used the "ZymoBIOMICS" mock community standard which also includes some 18S from ye…