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Hi huanle, I am very interested in the software developed by your group. I want to use the software to predict the methylation status for my own data, however, I do not have the training dataset. Coul…
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すでにDNA methylationをNanoporeで読む技術はかなり確立しています。
https://academic.oup.com/nar/article/47/8/e46/5356940
Direct RNA sequencingによるfull length sequenceやm6AなどのRNA modificationも難しいけど可能なようです。
https://www.na…
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Hi,
I used proovread to correct my Nanopore Direct RNA sequencing data with illumina sequencing data, **but the output .trimmed.f[aq] data is so small**. The ori long-reads fastq data is 788M, howeve…
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can proovread be used for ONT sequence. I am not sure if any parameter tuning might be required.
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Hi Heng :
Whether positive/negative strand should be considered when direct RNA sequencing reads are mapped to transcriptome(cDNA)?In my view, cDNA library is produced by reverse transcription of m…
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Hi:
I have successfully run the entire pipeline.But I found the **number of alternative splices** events detected by the pipeline is significantly **less than the annotation** file by comparing `re…
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Update the following URL to point to the GitHub repository of
the package you wish to submit to _Bioconductor_
- Repository: https://github.com/jipingw/DegNorm
Confirm the following by editing …
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Hi,
I will do some direct RNA sequencing soon & am trying to simulate the result in advance using trans-nanosim. I plan to use the data from [this article](https://www.nature.com/articles/nmeth.4577)…
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RNA-seq pipeline stall at this step
```
[2019-12-06T20:48Z] Counted 3476357 read pairs, 67679 single reads
[2019-12-06T20:48Z] Processed 9997901 reads in total
[2019-12-06T20:48Z] Cleanup of tempo…
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Hi:
1.
I find `basecalls.fa` that is generated by `basecall.py` and `model_final checkpoint` is completely unable to mapping to the reference sequence files(0% mapping ratio).
However,the …