-
Hi,
Many thanks for developing this great assembler.
I have recently updated to version 2.0 version of ABruijn and testing it out on relatively complex nanopore metagenomics samples. The eukary…
-
Hi,
I am testing how accurately I can correct reads from nanopore Minion by sequencing a plasmid.
I generated a VCF file by following all the steps given in this site. For the nanopolish variant …
-
Hello. I'm attempting to run a hybrid assembly with test input on a HTC cluster, I've ran SPAdes v3.11.1 a few times already on this cluster without nanopore and two sets of trusted contigs, so perhap…
-
Hi Ryan,
I'm processing a MinION dataset produced from an SQK-LSK108 kit and attempting to use Porechop to demux and trim adapters. It seems to be doing a reasonable job of identifying the adapters…
-
Hi all! I'm using MinION to teach myself bioinformatics for the first time so this issue is probably my ignorance rather than a bug.
I'm running canu v 1.6 on MacOS (laptop), trying to de-novo asse…
-
Forgive me if I have misread other issues about this. I tried https://github.com/marbl/canu/issues/607, but I'm not sure it was exactly addressed.
The error looks like:
-- Filtered list of reads f…
-
I'm running the following command
`canu -d ./Gene_Assembly -p Gene GenomeSize=7600 minReadLength=100 minOverlapLength=100 -nanopore-raw Merge_Output_File.fastq`
I'm getting error: `failed to cre…
-
Hi!
I would like to see the source code. Is that possible? In my situation, I really only need to see your code for aligning illumina reads to minION reads (using BLAT, if i've understood correctly…
-
I have a similar issue like #546 and #718
I installed canu 1.7 on an ubuntu (16.4) workstation. I have a 1G assembly length but with a very low nanopore reads coverage (5X).
This is the command I …
-
Dear all
I am trying to run canu for nanopore-raw sequences. I am running the pipeline on a high performance cluster via `qsub`
I get the following output:
```
-- This is canu parallel iteration #…