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I was mapping a subset of nanopore reads to a subset of a gfa, and everything worked fine under my previous minigraph version (0.15-r426) but then repeatedly failed an assertion in the latest version …
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toolshed.g2.bx.psu.edu/repos/iuc/unicycler/unicycler/0.5.0+galaxy1
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Hi - I'm using ratatosk 0.7.5 to correct nanopore long reads with some Illumina MiSeq data. Things seem to run fine for a few hours, and then terminates on the 2nd pass with
FileParser::FileParser(…
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Hi!
I have performed GWAS analysis using the unitigs approach in my dataset.
Is it possible to use for example the top 100 unitigs (with the lowest pvalue) to see how well they can predict my ph…
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What is happening at the scaffolding step, when the only input are two paired-end fastq Illumina files? According to the publication on Abyss v2, the information about paired ends of the same clusters…
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Hi,
Can `verkko` output the `noseq.gfa` for looking at the graph with bandage? For large genome, the sequence info cannot load into bandage easily.
baozg updated
2 years ago
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Hello,
First of all, this is really useful thanks for this tool.
I'm trying to use it on Hifiasm graphs. I saw issue#35 and while waiting for correct cigar as output of Hifiasm I implemented a s…
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Hello,
I would like to align PacBio HiFi reads to ONT reads with the goal of making a consensus of the alignment against the long read backbone (to correct the errors).
First, I wonder whether Win…
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Hello,
I have received an error message as following when ran "to_basespace --gfa example.unitigs.gfa --sequences example.sequences" with example data.
Could you give me some advice? Thanks!
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Hi there,
I've been trying to get STRONG to run on my universities HPC for awhile now. Installation has been pretty painful without a conda or working docker container to ease the process. It seems…