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This may be a naïve question as I'm new to ONT sequencing and analyses. What is the best approach to combine reads post simplex and duplex basecalling for genome assembly? I have a low number of duple…
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Hi,
I run
```
dorado basecaller dna_r10.4.1_e8.2_400bps_hac@v5.0.0 lambda7KM_Nano_proteinA_EcoGII.fast5/batch101.fast5 --modified-bases 6mA --trim --reference lambda.fa.fasta > test2.bam
```
a…
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# Issue Report
## Please describe the issue:
I have aligned/basecalled pod5->bams that I want to demultiplex into two files based on the presence/absence of a specific adapter sequence at the be…
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Hi everyone,
I currently running Guppy for the basecalling.
I found that the option '--fast5_out' was removed from Guppy 6.5.7.
Can anyone provide some information of why this option was not in…
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# Issue Report
## Please describe the issue:
The `sequencing_telemetry.js` file is not generated by Dorado. However, this file can still be generated using MinKNOW (Start > Analysis mode > Basec…
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Hej!
I am using the version methylartist 1.2.7, I have different result between region and locus graphs.
The bam files I am using have modification calls of 5hmC and 5mC in all context (guppy model …
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Hi @dvera , My fast5 file is produced in R7.3 flow cells using genomic sequencing kit SQK-MAP006, how can I set my CONFIG when I use "read_fast5_basecaller.py" for basecalling?
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It would be great if there was some better documentation explaining how to use `baseCalling_Matrix_*.pl` scripts.
For instance [baseCalling_Matrix_merger.pl](https://github.com/galaxy001/pirs/blo…
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For reasons that remain opaque to me, 00_preprocess.sh fails to complete after re-basecalling with Guppy _unless_ you specify the full path to the fast5 and fastq files. Unless I'm missing something, …
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Is https://github.com/nmiculinic/minion-basecaller/blob/master/mincall/example/example.model a well-trained model