-
-
Dear Developers, hello!
There are some projects using PacBio single-molecule long-read sequencing to analyze full-length transcriptome, but the raw data is in a bam type file and has to be changed in…
wu116 updated
2 months ago
-
Hello @rrwick,
I experienced some poor mapping performance with Minimap2 for reads simulated using the pacbio2016 (only changing depth, mean sequence identity, all other settings as default). I notic…
-
Hi,
I used the NextITS pipeline to analyze full-length ITS reads obtained through PacBio sequencing. However, I do not understand why the total number of reads in the OTU_table_LULU for each sample…
atfdh updated
1 month ago
-
Hi! I'm running long_read_typing.py for PacBio -reads for which I have extracted the HLA-regions (originally aligned data to chm13, then extracted hla with HLA*LA and ran bam2fastq). I was wondering a…
-
http://schatzlab.cshl.edu/data/skbr3/
-
Hi!
Do you have parameter recommendations for long accurate reads such as HiFi PacBio reads of ~15kb >Q20?
Thank you,
Armin
-
Other issues have already mentioned increased memory usage in the 3.4 version, but I have also observed some increased runtime and have run a quick comparison on one sample of mouse brain tissue with …
-
Hi ,
I would like to quantify the TE expression from pacbio iso-seq . I directly using minimap2 bam file to run TElocal using `--mode uniq`, but the ouput file all zero count. I would like to know it…
-
Hi,
Thanks for developing the package!
I have a few dozens of samples with captured PacBio hifi reads averaging 4kb in length but >200x coverage in a 1.6Mb human genome region, which has a lot o…