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It seems that falco doesn't handle reverse-complemented read sequences in mapped BAM files correctly.
Specifically, when flag bit 0x04 of a read is not set (i.e. when the read is mapped), falco shoul…
wm75 updated
4 months ago
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Hi,
Just a quick check question: In your manual here (https://warrenlab.github.io/agptools/) you say that flip will reverse-complement a sequence in the scaffold to re-orient a misassembled piece. …
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Hello, in my project I would like to just calculate the kmer counts for one side of the reads, read_1.fq.gz, for example.
And then afterwards, I would like to compare kmer.dump across samples, I wou…
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Firstly, thank you for `rust-bio`! I'm a new user, and enjoying it.
I just want to confirm that `bio::alignment::pairwise::Aligner` does not also align the reverse complement. Is there an aligner in …
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https://doi.org/10.1101/103663 (http://biorxiv.org/content/early/2017/01/27/103663)
> Deep learning approaches that have produced breakthrough predictive models in computer vision, speech recogniti…
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Hello!
I would love your opinion or advice with our method, and I have a few questions about how UMItools extract with a regex works.
I have nanopore long reads with dual 18bp UMIs, one on eac…
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hello,
I am running ragtag scaffold in order to fill in gaps between contigs using a reference genome. everything seems to run fine up until the very end when I get the error:
`raise RuntimeEr…
mabh5 updated
4 months ago
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## Expected Behavior
[dataset.zip](https://github.com/user-attachments/files/16023829/dataset.zip)
I have a group of sequences which is properly aligned with almost full length and >95% identity usi…
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Hi,
i'm wondering if it is neccessary to reverse all input DNA sequence to positive strand when using sei. Biologically speaking both strand should have the same peak but i'm not sure whether this is…