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I recently came across this bulletin from Illumina. https://www.illumina.com/content/dam/illumina/gcs/assembled-assets/marketing-literature/illumina-stranded-rna-t-overhang-tech-note-470-2020-010/ill…
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https://mp.weixin.qq.com/s/yuoNWk5dRlKp9SaJYCoe0Q
ixxmu updated
2 years ago
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Hi, I was wondering if I could run this program on bulk nanopore data?
I am trying to impute cpg sites to run a methylation atlas on cell free DNA data to determine cell of origin. Our data gives m…
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Hi, I recently learned to run megalodon with remora model, but came into an error that I don't know how to solve. From the log file, I don't see any error. But the output of the command is very worryi…
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I have been having trouble processing large data files that contain genome annotation information and gene ontology terms on my desktop RStudio when running statistical analysis for whole-genome bisul…
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Hi to all,
I am using Bismark (version 2.3.4.1) to analyze Bisulfite sequencing in paired-end unstranded samples. When I run Bismark using the following code:
`bismark --genome_folder [...] -1 …
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Hi,
I am trying to use bismark_genome_preparation in bismark via singularity to prepare the hg38 genome on the cluster, but I failed to launch the bowtier2 index at step3(See information below). And …
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Hello @heathsc,
I just pushed the bioconda version of gemBS up to 3.5.1 and I was testing out the installation using some of my own data and I hit an issue at the gemBS map step in the pipeline. I …
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Hi,
Thanks for the great tool.
I have used BISCUIT and generated bw and bam files. However, I am trying to calculate the bisulfite conversion rate. Is there any way so that BISCUIT can provide t…
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Hi,
Thanks for the great tool.
I have used BISCUIT and generated bw and bam files. However, I am trying to calculate the bisulfite conversion rate. Is there any way so that BISCUIT can provide th…