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Trying to annotate significant unitigs.
I annotated a genome (complete) using prokka then used the fasta and gff with annotate_hits_pyseer
this works and the script runs ok but there are no gene…
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Hello,
I run your workflow on 60cpu -- 500go RAM machine using slurm manager and a singularity install but my job crash at 50% of compleness.
The slurm.err file display the following error mess…
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Dear developers,
We have ~30x coverage of Nanopore PromethION data from a heterozygous invertebrate with a large genome (18Gbp). We also have approximately 30-40x coverage of 10X Chromium Illumina …
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Hello,
I am trying to merge two assemblies, but I get an error. Do you have an idea what can it be? o how can i understand the error?
quickmerge -d merge.canu.flye2.rq.delta -q canu/VdC07.all.fast…
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Could you easily produce GFAv1?
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see `sort_bcalm_unitigs` introduced in #310 as an example - this would involve creating an index of `{ contig_id: offset_in_bgzf_file }`
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Dear Dr. Ruan,
We intend to assemble the 2019-nCov virus genome with wtdbg2. Our sample contains 800x non PCR-free nanopore sequence. However, the program output empty .ctg.lay.gz file after multip…
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Hi, I'm new to canu and using it to process nanopore datas.
I ran the program with the default options (except that I specified the corMaxEvidenceErate at 0.15, since my target genome is GC reach and…
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Since we build an edge-centric de-Bruijn graph, there should be as many unitigs as edges divided by two. Since there are more edges, it might be that the removal of duplicates does not work correctly.…
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Hi all:
when I run this protocol, I get a error:
bedfile loaded
Starting Iteration 1
bedfile started
bedfile loaded
Loading Hi-C links
Finished loading Hi-C links, Loading unitig links now…