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Convert('GSE185224_clustered_annotated_adata_k10_lr0.92_v1.7.h5ad', "h5seurat",overwrite = TRUE,assay = "RNA")
seurat_obj
Biolz updated
6 months ago
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Hi!
I'm doing a project to do Differential gene expression on non-coding RNAs, so was planning to filter counts file only for non-coding probability.
But for that to happen, I'd need the read i…
idlip updated
2 months ago
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Hello, I have the error when running guidance = scglue.genomics.rna_anchored_guidance_graph(rna, atac) when using my dataset.
![image](https://github.com/gao-lab/GLUE/assets/72248550/d5b383e5-5efe-…
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I have two data sets of single-cell transcriptomes: snrna and sprna, where snrna was obtained under Seurat v4.3 and sprna was obtained under Seurat v5. I use the same code, as shown below.
```
W3.2…
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Hi @Griffan,
Just wondering whether there was precedent to use VB2 on RNA samples, or you can perceive any potential roadblocks?
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Hi, [Kallisto/BUSTools was recommended by Seurat](https://github.com/satijalab/seurat/issues/2813#event-3222404304) to perform RNA isoform/alternative splicing analysis. However, I didn't find the re…
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### Description of the bug
Experiencing 137 on samtools sort, after talking with slack community and seqeria, seems to be related to [this](https://github.com/nf-core/methylseq/pull/330)
### Command…
bc2zb updated
2 weeks ago
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Is there a specific requirement related to the normalization method for bulqRNA-seq data in immuCC? like TPM, FPKM, RPKM, or just count matrix of each gene.
Thank you!
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Dear Shi,
if I have a paired end small RNA-Seq data, can I analysis it using SPORTS?
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Hi, I have multiple bam files (test1.bam test2.bam test3.bam) and the genome fasta and annotation gff.
I want to visualize it with jigv. Could you please post the command for this.
Thanks