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I have downloaded a scRNA-seq matrix which has been clustered or named the cell types. Now I do not want to reanalyze this data, I just want to make the featureplot for several genes with seurat. I am…
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Hi,
I'm interested in integrating sc-RNA data sets and your package seems very suitable for this. However I get this error while running scMerge.
`sce_mESC_mg
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Dear Alex,
I used STAR to map RNA-seq to bacteria genome. When I build index,I only use fasta file without gtf file. The parameter "--genomeSAindexNbases" is 10. Then I start mapping step, it returns…
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Update the following URL to point to the GitHub repository of
the package you wish to submit to _Bioconductor_
- Repository: https://github.com/guldenolgun/NoRCE
Confirm the following by editin…
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Hello.
On a mac OS 10. I am stymied by the createIndices step
```
(snakePipes) x86_64-apple-darwin13% createIndices -v --local True --tempdir ~/Desktop/code/output-dir -o output-dir --genome f…
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Hello,
First, thanks for your package, it's been really useful for me.
I've been working with RNA-seq from _Saccharomyces cerevisiae_. I had a pipeline based on `edgeR` so I take the results from i…
matrs updated
4 years ago
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Hi,
First, thank you for creating Seurat!
Secondly, I am working to integrating multi-model single cell data from 4 different samples generated using the 10X platform. For each sample, I have ma…
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These are the datasets. Counts and initial clustering for all except MERFISH are in Dropbox.
- [x] single-cell SMART-seq (Allen)
- [x] single-nucleus SMART-seq (Allen)
- [x] single-cell 10X (Allen)…
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I am following workflow of '_Best-practices in single-cell RNA-seq: a tutorial_' to analyze my single-cell sequencing data sets.
I have calculated the size factor using the scran package and did not …
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I do 10x sc-RNA seq of monocyte and differentiated into osteoclast. The results can separate clearly monocyte, precursor, pre-osteoclast, osteoclast clusters. But I also see that the more osteoclastic…