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### Description of the bug
The `test_full`-profile doesn’t run out-of-the-box on scrnaseq v.2.7.1:
```
nextflow run nf-core/scrnaseq -r 2.7.1 -profile test_full,singularity --outdir results
...
…
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Checking out the steps ahead to align the trimmed sequences to an annotated genome using HISAT-2. Here are some resources I'm following:
- Steven Roberts FISH 546 course info: https://sr320.github.…
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hi，
I have a sample, and this sample has rnaseq data and isoseq data
braker.pl --genome=genome.fasta --prot_seq pro.fasta --bam=rnaseq.bam --bam=iso_seq.bam --threads 32
Is this command runni…
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Likely just clusters but very recent.
S:I203V appears to be a very rare mutations
even if it is predicted by Dadonaite and Bloom to boost binding on XBB backbone
Nearby mutations in that area as…
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If I pass a compressed fasta file to `--genome` I get the following error
```
$ iss generate -m miseq -g ref.fa.gz -n 50k -o iss_reads
INFO:iss.app:Starting iss generate
INFO:iss.app:Using kde E…
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Issues for the Book Genome Project will live at https://github.com/Open-Book-Genome-Project/sequencer/issues and the `epic` will be managed here on Open Library by @finnless
Issues:
- [x] #3…
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The number of ambiguous characters is often a good metric for genome quality. As far as I know, there's no way to add the number of counts of a given character to `seqkit stats`. I'm using `-G "Nn"` t…
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Hello everyone,
I'm trying to polish a large genome (3Gb) with Pilon but I'm having problems with RAM. I read that some people choose to split the genome to deal with RAM, so I would like to try th…
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Hi,
There are core genes listed in my gene_presence_absense.csv file that aren't included in my pan_genome_reference.fa file. All of the missing genes happen to be from the "group_" category. Of my…
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Hi, vg is a great software in pangenome.
I have 12 genome and gff files, and already built a graph-based pangenome with SV vcf file by _vg construct_ and _vg index_.
I also have some RNA-seq dat…