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For the following s/blow5 header made with blue-crab (0.1.2) , it does not seem that slow5tools degrade (1.3.0) recognizes the ULK kit.
```bash
#slow5_version 0.2.0
#num_read_groups 1
@a…
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Hi,
I am hoping to perform transcriptome assembly using both nanopore long read sequencing data and illumina short read sequencing data. It appears RATTLE only permits the use of long read sequenci…
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Hello,
I'm analysing single-cell full-length RNA sequencing data. I would like to perform an analysis on alternative splicing exons, which has been mentioned multiple times in your research group's…
weib3 updated
5 months ago
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It's OK In prod (e.g. here https://docs.lamin.ai/scrna#populate-metadata-registries-based-on-an-artifact)
I just saw it in a notebook from @sunnyosun
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# 运行代码
$ source ~/dnbc4tools/dnbc4tools2.1.3/sourceC4.bash
$ genome=~/dnbc4tools/GRCh38_gencode_v44_ref/
$ ~/dnbc4tools/dnbc4tools2.1.3/dnbc4tools rna run --name D2_1 \
> --cDNAfastq1 ./HRR1822706…
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Hello,
I am interested in direct-RNAseq data analysis. In the introduction, you have specified the used sequencing kit as "SQK-LSK108", which I believe is a cDNA or gDNA kit and not a dRNA kit. Cou…
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Discusses qPCR, RNA sequencing, single-cell RNA sequencing, and 16s rRNA Gene (DNA) sequencing.
# @grabear's Checklist
- [ ] Appropriate Content
- [ ] Well Formatted
- [ ] Working Links
- [ ]…
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1. A comparison of automatic **cell identification** methods for single-cell RNA sequencing data
Article Link: https://genomebiology.biomedcentral.com/articles/10.1186/s13059-019-1795-z
2. Challen…
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Hi there,
First of all, huge fan of this classification system, thank you so much for developing this!
I am currently using this tool on RNA sequencing data and was wondering if anyone has tried…
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So, I performed direct RNA sequencing on nanopore and got fast5 files for them. When I do tombo resquiggle, should me reference fasta file by DNA or RNA sequence in fasta?
For instance, if I sequen…