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Hello there,
I hope you're having an awesome day, and that all is going well on our side. I just wanted to reach out to you because I ran into an issue when installing/building cDNA_cupcake using t…
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Hi,
I tried to upload a rice cDNA FASTA file for reference, but failed with an error code below:
"Starting job.
Error: ErrorException: rename(/rnadetector/ws/storage/app/public/jobs/6//rnadete…
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code:
cut -f 1 -d ' ' ./SQANTI3_1_output/muscle_corrected.fasta > ./SQANTI3_1_output/cdna.new.fa
grep '^>' ./SQANTI3_1_output/cdna.new.fa | cut -d ' ' -f 1 | sed 's/^>//g' > /home/D…
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Hi,
During some comparisons of STAR and kallisto we saw that the number of genes/transcripts that STAR aligns to and outputs is much larger than kallisto. This obviously makes sense and is expected…
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we sequencing many ONT cDNA RNA data from different tissues, Can I use this software to analyze this data? Thanks
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I have tried to align the [cDNA reads]( http://s3.amazonaws.com/nanopore-human-wgs/rna/fastq/NA12878-cDNA_All_Guppy_3.2.6.fastq.gz) using minimap2 but only a small number of reads align to the human g…
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I met an error like this when I try to update me annotation using Launch_PASA_pipeline.pl, please help me fix it or give me some advice.
* [Fri Sep 15 01:56:05 2023] Running CMD: /home/MCY/App/PASApi…
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i am new to NGs analysis. i got an error while running trinity in conda which is:
Error encountered::
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Dear Mr/Mrs,
I am trying to combine data from two PacBio runs: DNA (10kb) + cDNA (10kb), these regions overlap each other partially and I would like to phase them based on short-tandem repeats and …
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Currently, we assume NM_007294.3 and NM_000059.3 for cdna coordinates coming from gnomad (see https://github.com/BRCAChallenge/brca-exchange/blob/master/pipeline/gnomad/gnomad_to_vcf.py#L103-L106). Is…